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single stranded rna sequencing strategy  (Roche)


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    Structured Review

    Roche single stranded rna sequencing strategy
    ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the <t>single-cell</t> <t>RNA-sequencing</t> dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.
    Single Stranded Rna Sequencing Strategy, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stranded+rna+sequencing+kits/pmc13030891-232-6-9?v=Roche
    Average 99 stars, based on 1 article reviews
    single stranded rna sequencing strategy - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "Nociceptor neurons control pollution-mediated neutrophilic asthma"

    Article Title: Nociceptor neurons control pollution-mediated neutrophilic asthma

    Journal: eLife

    doi: 10.7554/eLife.101988

    ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the single-cell RNA-sequencing dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.
    Figure Legend Snippet: ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the single-cell RNA-sequencing dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.

    Techniques Used: In Silico, Expressing, Gene Expression, Single Cell, RNA Sequencing



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    ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the <t>single-cell</t> <t>RNA-sequencing</t> dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.
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    ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the <t>single-cell</t> <t>RNA-sequencing</t> dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.
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    Flow chart of the study design and procedures. A total of 40 NSCLC patients were included in the cohort and all of them underwent the DNA-seq and FISH. Among them, 39 patients underwent WTS and 22 underwent targeted <t>RNA-seq.</t> DNA-seq, DNA <t>sequencing;</t> FISH, fluorescence in situ hybridization; NSCLC, non-small cell lung cancer; RET , rearranged during transfection; RNA-seq, RNA sequencing; WTS, whole-transcriptome sequencing.
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    Image Search Results


    ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the single-cell RNA-sequencing dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.

    Journal: eLife

    Article Title: Nociceptor neurons control pollution-mediated neutrophilic asthma

    doi: 10.7554/eLife.101988

    Figure Lengend Snippet: ( A ) In silico re-analysis of Artn expression in mouse immune cells using the ImmGen database . Artn and Ahr are expressed in Itgam + macrophages. Data are presented as per-gene z-scores of normalized gene expression, calculated by the median of ratios method. ( B ) In silico re-analysis of the single-cell RNA-sequencing dataset from (Sensoryomics; dbGaP accession phs001158) shows that Gfra3 is expressed in Trpa1 -positive nociceptors, C-LTMRs, and silent nociceptors within the human dorsal root ganglion. Expression levels are reported as per-gene z-scores calculated with the median-of-ratios normalization method.

    Article Snippet: Libraries were prepared using a poly(A)-enrichment, single-stranded RNA-sequencing strategy (KapaBiosystems, KAPA RNA Hyperprep Kit, #KR1352) and sequenced on an Illumina NextSeq500 platform with 75-cycle single-end reads.

    Techniques: In Silico, Expressing, Gene Expression, Single Cell, RNA Sequencing

    Flow chart of the study design and procedures. A total of 40 NSCLC patients were included in the cohort and all of them underwent the DNA-seq and FISH. Among them, 39 patients underwent WTS and 22 underwent targeted RNA-seq. DNA-seq, DNA sequencing; FISH, fluorescence in situ hybridization; NSCLC, non-small cell lung cancer; RET , rearranged during transfection; RNA-seq, RNA sequencing; WTS, whole-transcriptome sequencing.

    Journal: Translational Lung Cancer Research

    Article Title: Characterization of RET fusions via integrated DNA and RNA sequencing in early-stage non-small cell lung cancer: a retrospective study

    doi: 10.21037/tlcr-2025-702

    Figure Lengend Snippet: Flow chart of the study design and procedures. A total of 40 NSCLC patients were included in the cohort and all of them underwent the DNA-seq and FISH. Among them, 39 patients underwent WTS and 22 underwent targeted RNA-seq. DNA-seq, DNA sequencing; FISH, fluorescence in situ hybridization; NSCLC, non-small cell lung cancer; RET , rearranged during transfection; RNA-seq, RNA sequencing; WTS, whole-transcriptome sequencing.

    Article Snippet: For WTS, libraries were prepared utilizing the KAPA Stranded RNA Sequencing Library Preparation Kit (Roche, Basel, Switzerland).

    Techniques: DNA Sequencing, RNA Sequencing, Fluorescence, In Situ Hybridization, Transfection, Sequencing

    Distribution of RET fusion across different methods in the study cohort. (A) Distribution of different RET fusion groups identified by DNA-seq (n=40). (B) Oncoplot illustrating clinicopathologic and molecular characteristics of the study cohort. (C) Concordance among three different methodologies for the orthogonal validation of RET fusions (n=40). (D) Comparison of the consistency across the three methodologies. (E) The results of consistency of three methods. DNA-seq, DNA sequencing; FISH, fluorescence in situ hybridization; RET , rearranged during transfection; RNA-seq, RNA sequencing; WTS, whole-transcriptome sequencing.

    Journal: Translational Lung Cancer Research

    Article Title: Characterization of RET fusions via integrated DNA and RNA sequencing in early-stage non-small cell lung cancer: a retrospective study

    doi: 10.21037/tlcr-2025-702

    Figure Lengend Snippet: Distribution of RET fusion across different methods in the study cohort. (A) Distribution of different RET fusion groups identified by DNA-seq (n=40). (B) Oncoplot illustrating clinicopathologic and molecular characteristics of the study cohort. (C) Concordance among three different methodologies for the orthogonal validation of RET fusions (n=40). (D) Comparison of the consistency across the three methodologies. (E) The results of consistency of three methods. DNA-seq, DNA sequencing; FISH, fluorescence in situ hybridization; RET , rearranged during transfection; RNA-seq, RNA sequencing; WTS, whole-transcriptome sequencing.

    Article Snippet: For WTS, libraries were prepared utilizing the KAPA Stranded RNA Sequencing Library Preparation Kit (Roche, Basel, Switzerland).

    Techniques: DNA Sequencing, Biomarker Discovery, Comparison, Fluorescence, In Situ Hybridization, Transfection, RNA Sequencing, Sequencing